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Tools for preprocessing and normalizing results of MaxQuant experiments and their respective controls from a run by normalizing data from the output peptides.txt file.

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miano-fc/MaxQuant-Tools

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MaxQuant Tools

What's ✨new✨

Modified the program to allow users to specify how many experiments and controls they have

What I'm working on 🛠️

  • A GUI via tkinter
  • Streamlining the application/reducing overlapping code

Purpose

To compare results of two MaxQuant experiments and their respective controls from a run by normalizing data from the output peptides.txt file.

Functionality

Users can selected data by PEP and MS/MS count thresholds, proteins marked by MaxQuant as potential contaminats are removed by default.

Data is normalized by count and intensity columns: (Experiment 1 - Control 1, Experiment 2 - Control 2).

Output

  1. A .csv file containing aggregated, normalized data
  2. A .csv file containing differentially expressed proteins + log2 fold change of intensity
  3. .csv files containing proteins expressed in either Experiment 1 or Experiment 2

How to use

After uploading peptides.csv to the experimental_data folder, run tools through run_all_functions.py or individually.

Contributors

Michael Miano

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Tools for preprocessing and normalizing results of MaxQuant experiments and their respective controls from a run by normalizing data from the output peptides.txt file.

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