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Code for Perez Mojica et al., 2023, STAR Protocols

Prerequisites

  • Assumes a Linux or Mac environment and that you are working in the terminal.
  • For other software requirements, see publication.

Steps to reproduce results in paper

  1. Clone this repo and cd into the cloned folder.

  2. bash 01_dl_and_index_dm6.sh

  3. bash 02_dl_fqs_and_celseq_BCs.sh

  4. i. bash 03_run_star.sh sample1 SRR21697060_1.fastq.gz SRR21697060_2.fastq.gz 16

    ii. bash 03_run_star.sh sample2 SRR21697059_1.fastq.gz SRR21697059_2.fastq.gz 16

  5. Rscript --vanilla 04_make_counts_table.R

  6. Rscript --vanilla -e 'rmarkdown::render("05_example_ep.Rmd")'

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